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Journal of Electrochemical Science and Engineering, Vol. 4 No. 4, 2014.

Original scientific paper

https://doi.org/10.5599/jese.2014.0057

Characterization of an hrp-aox-polyaniline-graphite composite biosensor

Ana Carolina O. Santana ; Escola de Química, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, 2.030, Centro de Tecnologia, Bloco E, E-203, Cidade Universitária,CEP 21941-909, Rio de Janeiro, Brasil
Erica F. Southgate ; Escola de Química, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, 2.030, Centro de Tecnologia, Bloco E, E-203, Cidade Universitária,CEP 21941-909, Rio de Janeiro, Brasil
João Paulo B. G. Mendes ; Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, 2.030, Centro de Tecnologia, Bloco A, A-302, Cidade Universitária, CEP 21941-909, Rio de Janeiro, Brasil
Jo Dweck ; Escola de Química, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, 2.030, Centro de Tecnologia, Bloco E, E-203, Cidade Universitária,CEP 21941-909, Rio de Janeiro, Brasil
Eliana Mosse Alhadeff ; Escola de Química, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, 2.030, Centro de Tecnologia, Bloco E, E-203, Cidade Universitária,CEP 21941-909, Rio de Janeiro, Brasil
Ninoska Isabel Bojorge Ramirez ; Universidade Federal Fluminense, Dep. Engenharia Química e de Petróleo, R. Passo da Pátria, 156, Bl E-226, São Domingos , Niterói, CEP 24210-240, Rio de Janeiro, Brasil

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Abstract

Nowadays there is an increasing demand to develop new and robust biosensors in order to detect low concentrations of different chemicals, in practical and small devices, giving fast and confident responses. The electrode material was a polyaniline-graphite-epoxy composite (PANI/GEC). Alcohol oxidase (AOX) and horseradish peroxidase (HRP) enzymes were immobilized and the responses were tested by cyclic voltammetry. The conductivities for the composites of graphite/polyaniline were determined. The cyclic voltammograms allowed detecting ethanol in pure diluted samples in a range from 0.036 to 2.62 M. Differential scanning calorimetry (DSC) and thermal gravimetry analysis (TGA) were used to verify the thermal characteristics of the composites (0, 10, 20, 30 and 100 % of graphite). The Imax value was determined for the dual enzyme biosensor (0.0724 mA), and the Kapp m as 1.41 M (with R2 =0.9912).

Keywords

Cyclic voltammetry; ethanol; immobilized enzymes; PANI/GCE

Hrčak ID:

130214

URI

https://hrcak.srce.hr/130214

Publication date:

6.12.2014.

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