Original scientific paper

https://doi.org/10.5562/cca2046

Electroanalytical and HPLC Methods for the Determination of Oxcarbazepine in Spiked Human Urine and Tablet Dosage Form

Agnieszka Nosal-Wierciñska ; Faculty of Chemistry, Maria Curie-Sk³odowska University, 20-031 Lublin, Poland
Selehattin Yilmaz ; Canakkale Onsekiz Mart University, Faculty of Science and Arts, Department of Chemistry, 17020, Canakkale, Turkey
Sevcan Binel ; Canakkale Onsekiz Mart University, Faculty of Science and Arts, Department of Chemistry, 17020, Canakkale, Turkey
Sultan Yagmur ; Canakkale Onsekiz Mart University, Faculty of Science and Arts, Department of Chemistry, 17020, Canakkale, Turkey
Gulsen Saglikoglu ; Canakkale Onsekiz Mart University, Faculty of Science and Arts, Department of Chemistry, 17020, Canakkale, Turkey
Murat Sadikoglu ; Gaziosmanpasa University, Faculty of Education, Department of Science Education, 60100, Tokat, Turkey
Mustafa Yıldız ; Canakkale Onsekiz Mart University, Faculty of Science and Arts, Department of Chemistry, 17020, Canakkale, Turkey

Abstract

In this study, the electrochemical reduction and determination of oxcarbazepine were easily
realized in various buffer solutions in the pH range of 4.50 to 11.15 in real samples using glassy car-bon electrode (GCE) by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) tech-niques. The influence of pH on the cathodic peak current and peak potential was investigated. Scan rate studies were also completed. The diffusion controlled nature of the peak was established. The best results for the quantitative determination of oxcarbazepine were obtained by DPV technique in Britton-Robinson (BR) buffer (pH 8.05). In this basic medium, one irreversible and sharp cathodic peak was observed. A linear
calibration curve for DPV analysis was constructed in the oxcarbazepine concentration range of 8×10–6 to 1×10–4 M. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 1.65×10–6 and 5.51×10–6 M, respectively. Repeatability, reproducibility, precision and accuracy of the developed
technique were checked in spiked human urine and tablet dosage forms by recovery studies and re-sults of the high performance liquid chromatography (HPLC) technique. A reduction mechanism for the electrode reaction was proposed.

Keywords

oxcarbazepine; human urine; dosage form; electrochemical reduction; determination; high performance liquid chromatography (HPLC)

Hrčak ID:

130428

URI

https://hrcak.srce.hr/130428

Publication date:

18.11.2014.

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