Original scientific paper

Quantitative Determination of Pesticides in Soil by Thin-layer Chromatography and Video Densitometry

Mira Petrović ; Faculty of Chemical Engineering and Technology, Laboratory of Analytical Chemistry, Marulićev trg 19, Zagreb, Croatia
Sandra Babić ; Faculty of Chemical Engineering and Technology, Laboratory of Analytical Chemistry, Marulićev trg 19, Zagreb, Croatia
Marija Kaštelan-Macan ; Faculty of Chemical Engineering and Technology, Laboratory of Analytical Chemistry, Marulićev trg 19, Zagreb, Croatia

Abstract

Reversed phase thin-layer chromatography (RP-TLC) in conjunction with video densitometry has been used for the quantitative determination of a six-component mixture of pesticides. Excellent separation of propham, chlorpropham, atrazine, diflubenzuron, tetramethrin and α-cypermethrin was achieved using the methanol/water solvent system (volume fraction of methanol, φ = 80%). Video densitometric quantification was validated for linearity, precision and detection limit. All results were satisfactory according to the validation requirements. The method was tested for the determination of pesticides from spiked soil using ultrasonic extraction with various solvents.

Keywords

thin-layer chromatography; video densitometry; pesticide; soil

Hrčak ID:

132090

URI

https://hrcak.srce.hr/132090

Publication date:

1.2.2000.

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