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Original scientific paper

DETECTION OF RENIBACTERIUM SALMONINARUM IN TISSUE OF BROOK TROUT (SALVELINUS FONTINALIS) BY NESTED RT–PCR

I. Vardić
D. Kapetanović
D. Valić
B. Kurtović
Z. Teskeredžić
E. Teskeredžić


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Abstract

Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in
brook trout tissue with gross clinical signs of disease by nested RT–PCR. Determination of partial sequence of bacterial msa gene was done prior to comparison with similar sequences from different R. salmoninarum isolates. Nested RT–PCR proved to be a rapid and valuable diagnostic tool for R. salmoninarum detection, and sequence analysis confirmed previously reported genetic uniformity of this bacteria.

Keywords

Renibacterium salmoninarum; nested RT–PCR; msa gene sequence; brook trout

Hrčak ID:

13450

URI

https://hrcak.srce.hr/13450

Publication date:

18.4.2007.

Article data in other languages: croatian

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