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Original scientific paper

https://doi.org/10.1515/acph-2015-0024

Dissolution profiles of perindopril and indapamide in their fixed-dose formulations by a new HPLC method and different mathematical approaches

ANNA GUMIENICZEK ; Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University in Lublin, 20-090 Lublin, Poland
PAULINA MĄCZKA ; Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University in Lublin, 20-090 Lublin, Poland
ŁUKASZ KOMSTA ; Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University in Lublin, 20-090 Lublin, Poland
RAFAŁ PIETRAŚ ; Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University in Lublin, 20-090 Lublin, Poland


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Abstract

A new HPLC method was introduced and validated for simultaneous determination of perindopril and indapamide. Validation procedure included specificity, sensitivity, robustness, stability, linearity, precision and accuracy. The method was used for the dissolution test of perindopril and indapamide in three fixed-dose formulations. The dissolution procedure was optimized using different media, different pH of the buffer, surfactants, paddle speed and temperature. Similarity of dissolution profiles was estimated using different model-independent and model-dependent methods and, additionally, by principal component analysis (PCA). Also, some kinetic models were checkedwith dissolved amounts of drugs as a function of time.

Keywords

perindopril; indapamide; dissolution profiles; model-independent methods; model-dependent methods; PCA

Hrčak ID:

134867

URI

https://hrcak.srce.hr/134867

Publication date:

30.9.2015.

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