Acta Pharmaceutica, Vol. 65 No. 3, 2015.
Original scientific paper
https://doi.org/10.1515/acph-2015-0026
Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry
SVJETLANA LUTEROTTI
orcid.org/0000-0001-8698-495X
; University of Zagreb, Faculty of Pharmacy and Biochemistry, HR-10000 Zagreb, Croatia
TONČICA VUKMAN KORDIĆ
; Agency for Medicinal Products and Medical Devices, HR-10000 Zagreb, Croatia
SLAVICA DODIG
orcid.org/0000-0002-3419-5171
; Children´s HospitalSrebrnjak, HR-10000 Zagreb, Croatia
Abstract
In an attempt to provide a reliable status of metal ions in children, new methods of analysis of children's sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dm–3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated against reference methods. Accuracy, sensitivity and precision have proven them to be comparable to the reference methods in terms of analytical performance, and applicable to analyses of children's sera.
Keywords
copper; zinc; FAAS; single-step analysis; children's serum
Hrčak ID:
134869
URI
Publication date:
30.9.2015.
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