Essays

Simultaneous plasmid integration: a unifying model of multiple plasmid integration into the yeast chromosome

PETAR T. MITRIKESKI orcid.org/0000-0002-8859-3490 ; 1_Laboratory for Evolutionary Genetics Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia; 2_Institute for Research and Development of Sustainable Ecosystems, Ivana Lučića 5 (CTT-FSB) HR-10000, Zagreb, Croat
ANA ŠIMATOVIĆ ; Laboratory for Evolutionary Genetics Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
KRUNOSLAV BRČIĆ-KOSTIĆ ; Laboratory for Evolutionary Genetics Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia

Abstract

Recombination of non-replicative plasmids bearing yeast homology with
the chromosome can integrate the plasmid molecule into the genome. Such process is also known to integrate more than one plasmid molecule leading to multiple, tandem plasmid integration. However, its exact molecular mechanism remains unknown. There are two alternative models to explain such integration. The first predicts single integration of a super-plasmid molecule and the second sequential integration of several independent molecules, but neither is able to comprehend all experimental data. Therefore, here is presented a theoretical model that unifies both prior models owing to the possibility that two plasmid molecules recombine with the chromosome simultaneously. This model was used as a theoretical tool in order to discriminate between existing alternatives extracting the sequential model as a better overall explanation.

Keywords

Saccharomyces; Homologous recombination; Multiple plasmid integration; Genetic model

Hrčak ID:

135147

URI

https://hrcak.srce.hr/135147

Publication date:

30.9.2014.

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