Original scientific paper

Novel 57 kDa Glycoprotein in the Sera of Humans under Stress

Mima Flögel ; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Gordan Lauc ; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Tihana Žanić-Grubišić ; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Jerka Dumić ; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Karmela Barišić ; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia

Abstract

Serum glycoproteins from individuals under chronic stress (prisoners of war from detention camps, n - 30) were analyzed by the Western-blot method applying a set of five different lectins (DSA,
GNA, MAA, PNA and SNA). Glycoprotein patterns of samples from the stressed group revealed significant changes if compared to the control sera (from apparently non-stressed volunteers): both galactose-specific DSA lectin and sialic acid-specific SNA lectin recognized a novel nonconsitutional glycoprotein with an apparent size of 57 kDa in the stress group, which was completely absent in the control group. In addition, concentration of a 45 kDa SNA reactive glycoprotein increased more than 500 fold. The high incidence of disturbed patterns of glycoproteins in the sera of stressed subjects (95%) points to their important role in stress response and qualifies them for widespread testing as potential diagnostic markers of the stress syndrome.

Keywords

Hrčak ID:

135969

URI

https://hrcak.srce.hr/135969

Publication date:

4.3.1996.

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