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Conference paper

Interaction of the Escherichia coli host and phage λ: Role of the RecBCD-Gam complex

Željko Trgovčević ; Ruđer Bošković Institute, HR-10 000 Zagreb, Croatia
Erika Salaj-Šmic ; Ruđer Bošković Institute, HR-10 000 Zagreb, Croatia


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Abstract

Biochemical experiments have suggested that the Gam protein from phage X inhibits all the catalytic activities of the RecBCD enzyme of Escherichia coli. The genetic data are at variance with these experiments. The RecBCD-binding Gam protein has profound effects on the physiology of phage X and its host. These effects cannot be explained by the inhibitory action of Gam; the RecBCD-Gam complex apparently retains certain enzymatic activities. The mechanism by which its activities aid a substantial fraction of the phage to escape the EcoK restriction is proposed.

Keywords

Hrčak ID:

136718

URI

https://hrcak.srce.hr/136718

Publication date:

1.9.1995.

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