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Croatian Medical Journal, Vol. 56 No. 1, 2015.

Original scientific paper

https://doi.org/10.3325/cmj.2015.56.4

Ginkgolide B increases hydrogen sulfide and protects against endothelial dysfunction in diabetic rats

Guo-Guang Wang ; Department of Pathophysiology, Wannan Medical College, Wuhu, People’s Republic of China
Qing-Ying Chen ; Command, Jinan, People’s Republicof ChinaGeneral Hospital of Jinan Military
Wei Li ; Department of Pathophysiology, Wannan Medical College, Wuhu, People’s Republic of China
Xiao-Hua Lu ; Department of Pathophysiology, Wannan Medical College, Wuhu, People’s Republic of China
Xue Zhao ; Department of Pathophysiology, Wannan Medical College, Wuhu, People’s Republic of China

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Abstract

Aim To evaluate the effect of ginkgolide B treatment on
vascular endothelial function in diabetic rats.
Methods The study included four groups with 15 male
Sprague-Dawley rats: control group; control group treated
with ginkgolide B; diabetic group; and diabetic treated
with ginkgolide B. The activity of superoxide dismutase
(SOD), malondialdehyde content, and nicotinamide adenine
dinucleotide phosphate (NADPH) oxidase subunits,
and glutathione peroxidase 1 (GPX1) protein expression
were determined in aortic tissues. Vasoconstriction to phenylephrine
(PHE) and vasorelaxation to acetylcholine (Ach)
and sodium nitroprusside (SNP) were assessed in aortic
rings. Nitric oxide (NO) and hydrogen sulfide (H2S) were
measured, as well as cystathionine γ lyase (CSE) and cystathionine
β synthetase (CBS) protein expression, and endothelial
nitric oxide synthase (eNOS) activity.
Results Diabetes significantly impaired PHE-induced vasoconstriction
and Ach-induced vasorelaxation (P < 0.001),
reduced NO bioavailability and H2S production (P < 0.001),
SOD activity, and GPX1 protein expression (P < 0.001), and
increased malondialdehyde content and NADPH oxidase
subunits, and CSE and CBS protein expression (P < 0.001).
Ginkgolide B treatment improved PHE vasoconstriction
and Ach vasorelaxation (P < 0.001), restored SOD (P = 0.005)
and eNOS (P < 0.001) activities, H2S production (P = 0.044)
and decreased malondialdehyde content (P = 0.014). Vasorelaxation
to SNP was not significantly different in control
and diabetic rats with or without ginkgolide B treatment.
Besides, ginkgolide B increased GPX1 protein expression
and reduced NADPH oxidase subunits, CBS and CSE protein
expression.
Conclusion Ginkgolide B alleviates endothelial dysfunction
by reducing oxidative stress and elevating NO bioavailability
and H2S production in diabetic rats.

Keywords

Hrčak ID:

139291

URI

https://hrcak.srce.hr/139291

Publication date:

15.2.2015.

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