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Croatian Medical Journal, Vol. 56 No. 2, 2015.

Original scientific paper

https://doi.org/10.3325/cmj.2015.56.85

Cellobiose-coated poly(lactideco- glycolide) particles loaded with diphtheria toxoid for per os immunization

Tetiana Chudina ; Palladin Institute of Biochemistry of the National Academy ofSciences of Ukraine (NASU), Kyiv,Ukraine
Andrii Labyntsev ; Palladin Institute of Biochemistry of the National Academy ofSciences of Ukraine (NASU), Kyiv,Ukraine
Kyrylo Manoilo ; Palladin Institute of Biochemistry of the National Academy ofSciences of Ukraine (NASU), Kyiv,Ukraine
Denys Kolybo ; Educational and Scientific Center “Institute of Biology,” Taras Shevchenko National University inKyiv, Kyiv, Ukraine
Serhiy Komisarenko ; Palladin Institute of Biochemistry of the National Academy ofSciences of Ukraine (NASU), Kyiv,Ukraine

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Abstract

Aim To evaluate the dose-dependent immunogenic properties
of poly(lactide-co-glycolide) (PLGA) particles coated
with cellobiose as antigen carriers for oral immunization.
Methods Two types of PLGA-cellobiose particles (PLGAcellobiose-
1, ~ 0.8 μm and PLGA-cellobiose-2, ~ 1.2 μm)
containing non-toxic recombinant subunit B (SbB) of diphtheria
toxin fused with enhanced green fluorescent protein
were characterized in vitro for their size, shape, antigen
loading, and ability to induce phagocytosis. Different doses
of antigen, immobilized on the particles (2.5 μg, 25 μg,
250 μg, and 2500 μg per 1 kg of body weight), were administered
per os 3 times with intervals of 2 weeks to BALB/c
female mice. The antigen-specific IgG and IgA antibodies
were estimated in serum by ELISA.
Results After the first immunization, increase in concentration
of blood antitoxic antibodies was detected. Antigen
dose 250 μg/kg was the most immunogenic for IgG
antibodies induction for both types of PLGA-cellobiose
particles. Antigen doses 25 μg/kg and 2.5 μg/kg were the
most immunogenic for IgA antibodies induction by PLGA-
cellobiose 1 and 2 particles, respectively. The second
and the third treatment had no significant effect on the
immune response or even reduced it, which could be explained
by immune tolerance induction by the antigens
delivered per os.
Conclusion Our results suggest that the correct dose of
PLGA-cellobiose particles loaded with antigen could significantly
increase the humoral immune response against
the introduced antigen already after the first immunization.
Thus, PLGA particles can be considered as a potent
component of oral vaccines.

Keywords

Hrčak ID:

139299

URI

https://hrcak.srce.hr/139299

Publication date:

15.4.2015.

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