Original scientific paper

Preparation of microspheres containing leptospiral antigen using biodegradable alginate polymers.

Fatemeh Inanlou ; Department of Microbiology, Islamic Azad University, Karaj Branch, Karaj, Iran
Pejvak Khaki ; Leptospira Reference Laboratory, Department of Microbiology, Razi Vaccine and Sera Research Institute, Karaj, Iran
Naser Mohammadpour ; Department of Venom and Human Sera, Razi Vaccine and Sera Research Institute, Karaj, Iran
Soheila Moradi Bidhendi ; Leptospira Reference Laboratory, Department of Microbiology, Razi Vaccine and Sera Research Institute, Karaj, Iran
Hossein Zolfagharian ; Department of Venom and Human Sera, Razi Vaccine and Sera Research Institute, Karaj, Iran
Yung-Fu Chang ; Department of Population, Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
Mohammad R. Bazrafshan ; Alborz University of Medical Sciences, Karaj, Iran
Forough Saraei ; Department of Microbiology, Islamic Azad University, Karaj Branch, Karaj, Iran

Abstract

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. Although the currently available whole cell leptospiral vaccines can induce protection against Leptospirosis, further study for a new generation of vaccine that can stimulate long-term immunity is needed. Biodegradable microspheres as antigen delivery systems have been extensively investigated for decades, especially those based on hydrophilic polymers, such as alginate and chitosan, which have excellent biocompatibility, non-toxicity and biodegradability. The aim of this study was to prepare and characterize alginate microspheres as an antigen delivery system for immunization against leptospirosis. Alginate microspheres containing Leptospiral antigen (LA) were prepared by an emulsification method and characterized for shape, size distribution, loading efficiency (LE), loading capacity (LC) and release profie. The effects of some parameters (such as concentration of alginate and emulsifiers and stirring rate) on microspheres characteristics were investigated. The optimal condition parameters for the preparation of LA loaded alginate microspheres were estimated. The optimum concentrations were obtained for alginate and emulsifiers, 3.5 % (w/v), span 80 (0.2 % w/v) and tween 80 (3.75 % w/v), respectively. Moreover, appropriate homogenizing rate was obtained at 500 rparticle size of the microspheres as 200 μm, loading efficiency 97 % and loading capacity 8 %. A suitable release profile was observed for in vitro release test of LA from alginate microspheres over an extended period of time (192 hours). These results make the alginate microspheres particularly interesting for an LA delivery system.pm. Our results showed the mean particle size of the microspheres as 200 μm, loading effiiency 97 % and loading capacity 8 %. A suitable release profile was observed for in vitro release test of LA from alginate microspheres over an extended period of time (192 hours). These results make the alginate microspheres particularly interesting for an LA delivery system.

Keywords

Leptospira spp.; leptospiral antigen; alginate,; microspheres; antigen delivery; immunization

Hrčak ID:

145176

URI

https://hrcak.srce.hr/145176

Publication date:

2.9.2015.

Article data in other languages: croatian

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