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Croatica Chemica Acta, Vol. 88 No. 4, 2015.

Original scientific paper

https://doi.org/10.5562/cca2720

Surface-enhanced Raman Scattering Enhancement Factors for RNA Mononucleotides on Silver Nanoparticles

Snežana Miljanić orcid id orcid.org/0000-0003-1179-2253 ; Division of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Marina Ratkaj ; PLIVA Croatia, Teva Pharmaceutical Industries Ltd., Research and Development, Prilaz baruna Filipovića 29, HR-10000 Zagreb, Croatia
Igor Avdejev ; PLIVA Croatia, Teva Pharmaceutical Industries Ltd., Research and Development, Prilaz baruna Filipovića 29, HR-10000 Zagreb, Croatia
Karlo Meglić ; Division of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Adriana Kenđel ; Division of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

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Abstract

Surface-enhanced Raman scattering (SERS) enhancement factors (EF) were evaluated for RNA mononucleotides: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), cytidine 5'-monophosphate (CMP) and uridine 5'-monophosphate (UMP), on silver nanoparticles, which differed in shape (nanospheres, nanostars) and stabilizing anionic layer (chlorides, citrates) on the metal surface. In freshly prepared silver colloids the enhanced Raman scattering was observed for all the RNA mononucleotides on the chloride coated silver nanospheres, Ag_Cl nsp (EF ≈ 104), for AMP only on the citrate coated silver nanospheres, Ag_cit nsp (EF ≈ 103), while not obtained at all for any of the mononucleotides on the citrate stabilized silver nanostars, Ag_cit nst. Upon aggregation, the SERS activity of all the silver colloids increased, whereby the purine mononucleotides, AMP and GMP, more strongly scattered radiation on Ag_Cl nsp, and the pyrimidine mononucleotides, CMP and UMP, on Ag_cit nsp. Regardless of the silver nanoparticles, the higher EFs were evaluated for AMP and GMP (EF up to 5 × 106), than for CMP and UMP (EF ≈ 5 × 104).

Keywords

surface-enhanced Raman scattering; enhancement factor; RNA mononucleotide; silver nanoparticles; shape; anions

Hrčak ID:

150554

URI

https://hrcak.srce.hr/150554

Publication date:

31.12.2015.

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