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Croatian Medical Journal, Vol. 56 No. 6, 2015.

Editorial

https://doi.org/10.3325/cmj.2015.56.515

1,25-hydroxyvitamin D relieves colitis in rats via downregulation of toll-like receptor 9 expression

Zhang-han Dai ; Department of Gastroenterology, Peking Union Medical CollegeHospital, Chinese Academy ofMedical Science and Peking UnionMedical College, Beijing, China
Bei Tan ; Department of Gastroenterology, Peking Union Medical CollegeHospital, Chinese Academy ofMedical Science and Peking UnionMedical College, Beijing, China
Hong Yang ; Department of Gastroenterology, Peking Union Medical CollegeHospital, Chinese Academy ofMedical Science and Peking UnionMedical College, Beijing, China
Ou Wang ; Department of Endocrinology Key Laboratory of Chinese Health Ministry, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
Jia-Jiaming Qian ; Department of Gastroenterology, Peking Union Medical CollegeHospital, Chinese Academy ofMedical Science and Peking UnionMedical College, Beijing, China
Hong Lv ; Department of Gastroenterology, Peking Union Medical CollegeHospital, Chinese Academy ofMedical Science and Peking UnionMedical College, Beijing, China

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Abstract

Aim To investigate the therapeutic and immunoregulatory
effects of 1,25-dihydroxyvitamin D (1,25(OH)D3) on 2,4,6-
trinitrobenzenesulfonic acid (TNBS) -induced colitis in rats.
Methods Experimental colitis induced by enema administration
of TNBS plus ethanol was treated with 5-aminosalicylic
acid (5-ASA) and/or 1,25(OH)D3. Disease activity was
measured using the disease activation index (DAI), colon
macroscopic damage index (CMDI), histological colonic
damage score, and myeloperoxidase (MPO) activity. The
expression of toll-like receptor 9 (TLR9) in the colon was
determined by reverse transcription-polymerase chain reaction
and immunohistochemistry.
Results Rats with TNBS-induced colitis had significantly elevated
DAI, CMDI, histological colonic damage score, and
MPO activity (all P < 0.001) compared to rats without colitis.
Treatment with 5-ASA or 1,25(OH)D3 ameliorated colitis
by lowering CMDI (P = 0.049, P = 0.040, respectively), histological
colonic damage score (P = 0.010, P = 0.005, respectively),
and MPO activity (P = 0.0003, P = 0.0013, respectively)
compared with the TNBS group. Combined treatment
with 5-ASA and 1,25(OH)D3 significantly decreased MPO
activity (P = 0.003). 1,25(OH)D3 attenuated colitis without
causing hypercalcemia or renal insufficiency. TNBS significantly
increased the number of TLR9 positive cells compared
to control (P < 0.010), while 5-ASA, 1,25(OH)D3, and
combined treatment with 5-ASA and 1,25(OH)D3 significantly
decreased it compared to TNBS group (all P < 0.010).
In TNBS group a moderate correlation was observed between
MPO activity and the number of TLR9-positive cells
(r = 0.654, P < 0.001).
Conclusion TLR9 expression correlates with the extent of
inflammation in TNBS-induced colitis. 1,25(OH)D3 relieves
this inflammation possibly by decreasing TLR9 expression.

Keywords

Hrčak ID:

153074

URI

https://hrcak.srce.hr/153074

Publication date:

15.12.2015.

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