Professional paper
Determination of heavy metals in biological material with Chelex 100 resin
D.V. Djarmati
; Public Health Institute, Belgrade, Yugoslavia
M.K. Stanković
; Institute of Occupational and Radiological Health, Belgrade, Yugoslavia
Abstract
Own results of the determination of several metals in urine are presented. The samples were pretreated using the wet digestion procedure and concentrated by passing through a column filled with the chelating resin Chelex 100. After elution with diluted nitric acid, the same sample was used for the determination of lead, cadmium, copper, zinc and nickel. The metals eluted by this procedure can be determined with any instrumental technique available. For this study the method of atomic absorption spectrophotometry (AAS) was chosen. The best results were obtained at pH 6.0. Under the above mentioned conditions full chelation and elution from the column were achieved for all the metals determined. Being highly reproducible the method is applicable m everyday practice. Heavy metal content in biological material (urine, blood, hair, tissues) is an important parameter of human exposure in the working and living environment. Usually, it is very low, except in accidental or long-lasting exposures when even untoward clinical manifestations may become apparent.
Keywords
Hrčak ID:
153307
URI
Publication date:
8.10.1987.
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