Original scientific paper

Immunohistochemical detection of bovine herpesvirus type 1 and bovine adenovirus type 3 antigens in frozen and paraffinized lung sections of pneumonic sheep and goats.

Ali O. Çeribasi ; De partment of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey
Songül Çeribasi ; De partment of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey
Mustafa Ozkaraca ; Departme nt of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

Abstract

The aim of this study was to determine Bovine Adenovirus type 3 (BAV3) and Bovine Herpesvirus type 1 (BHV1) antigens in frozen and formalin-fixed paraffin-embedded lung tissues of pneumonic sheep and goats, using direct fluorescent a ntibody technique (DFAT) and immunoperoxidase (IP ) staining. For this purpose, the lungs of 1276 male sheep and 1087 male goats, which were raised in various farms in the Elazig province and surrounding areas and were brought to the abattoir for slaughtering between January and December 2011, were examined. Macroscopic pneumonia findings were detected only in the apical and cardiac lobes of the lungs of 132 sheep and 145 goats. The rates of mild, moderate and severe consolidations observed in the apical and cardiac lobes of pneumonic lungs were 57.5 %, 29.5 % and 12.8 % for sheep, respectively and 62.0 %, 20.0 % and 17.9 % for goats, respectively. Pneumonias were microscopically classified in sheep and goats as catarrhalpurulent (40.1 % and 33.1 %), interstitial (26.5 % and 31.7 %), fibrinous (21.2 % and 26.9 %), verminous (9.4 % and 6.2 %) and pulmonary adenomatosis (1.5 % and 2.0 %). In the examination with IP and DFAT in terms of BAV3 and BHV1 antige ns, a total of 249 (116 sheep and 133 goats) pneumonic lungs, but not verminous and pulmonary adenomatosis, were considered. BAV3 and BHV1 antigens were determined to be 5.1 % and 1.7 % in sheep and 0.7 % and 2.2 % in goats by the IP method, respectively. BAV3 and BHV1 antigens were found to be 9.4 % and 5.1 % in sheep and 4.5 % and 7.5 % in goats by DFAT, respectively. In conclusion, the presence of viral antigens in lung tissues of sheep and goats may indicate that natural pneumonia may be induced by BAV3 and BHV1 or possibly other species-specific adenoviruses and herpesviruses. In addition, it is thought that sheep and goats might have a role in the transmission of these viruses to cattle.

Keywords

bovine herpesvirus 1; bovine adenovirus 3; sheep; goat; pneumonia; immunofluorescence; immunoperoxidase

Hrčak ID:

153489

URI

https://hrcak.srce.hr/153489

Publication date:

15.2.2016.

Article data in other languages: croatian

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