Original scientific paper

Superovulatory response in relation to the size and side of ovary location in high yielding dairy cows on the first day of treatment protocol.

Giedrius Palubinskas ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Vytuolis Žilaitis ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Vida Juozaitienė ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Lina Laučienė ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Aurimas Gavelis ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Antanas Sederevičius ; Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania

Abstract

The aim of this research was to determine the relation between the size of ovaries, their topographic position, ovarian structures (follicles (F) and corpora lutea (CL)) before superovulatory treatment and the superovulatory response and embryo yield. Holsteinized dairy cows (32) were treated for superovulation with Pluset® (Laboratorios Calier, S.A., Barselona, Spain). On Day 1 of the superovulatory treatment and during the procedure to recover the embryos, we examined the ovaries of donors with an ultrasound scanner. The ovaries were divided into 3 groups according to their size. Group 1 ovaries (<668.0 mm2) were more than 25 % smaller and Group 3 ovaries (>1112.0 mm2) were 25 % larger than Group 2 ovaries, the area of which varied from 668.0 to 1112.0 mm2 (mean, 890.0 mm2 ± 25 %). After superovulation, the mean area of the right side ovaries (RSO) (3147.61 ± 106.6 mm2) was by 27.3 % (P<0.05) larger than the mean area of the left side ovaries (LSO). Irrespective of location, the ovaries after superovulation increased 3.2 times; however, the increase in the RSO, except for Group 3, was 14 % more intense (P>0.05) compared with the LSO. The most intense response to the superovulatory treatment by ovary size was observed in Group 1 ovaries: the RSO increased by 5.5 times and the LSO by 4.8 times, but according to the absolute measure, the greatest increase was observed in Group 3, where ovaries on both sides increased 2.4 times. According to the number of CL, Group 1 RSO donors showed a 2.3 times (P<0.05) weaker response to the superovulatory treatment in comparison with Group 3 RSO donors. The number of embryos recovered (9.8 ± 2.2) was 3.6 times (P<0.05) higher among the donors with Group 3 RSO before superovulation, compared with the donors’ Group 1 RSO (2.7 ± 0.5). The number of transferable embryos was 3.2 times (P<0.05) higher in cows with Group 3 (6.3 ± 0.6) RSO in comparison with the donors’ Group 1 (2.0 ± 0.3) RSO. Corpora lutea in the RSO demonstrated a significant impact of 85 % (P<0.05) on the yield of embryos, compared with only 30 % (P<0.05) for the LSO.

Keywords

donors; cows; superovulatory treatment; superovulatory response; embryo yield

Hrčak ID:

153493

URI

https://hrcak.srce.hr/153493

Publication date:

15.2.2016.

Article data in other languages: croatian

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