Original scientific paper

Effects of three-week zinc and melatonin supplementation on the oxidant-antioxidant system in experimental renal ischemia-reperfusion in rats

Mine Yilmaz ; Selcuk University, Medical School, Department of Physiology, Konya, Turkey
Rasim Mogulkoc orcid.org/0000-0001-6155-6780 ; Selcuk University, Medical School, Department of Physiology, Konya, Turkey
Abdulkerim Kasim Baltaci ; Selcuk University, Medical School, Department of Physiology, Konya, Turkey

Abstract

Renal ischemia-reperfusion directly affects glomerular and tubular epithelium. Oxygen free radicals have a significant part in the pathophysiology of renal ischemia-reperfusion injury. The present study aimed to identify the effects of 3-week zinc, melatonin, and zinc + melatonin supplementation on malondialdehyde (MDA) levels in tissue and plasma and glutathione levels (GSH) in erythrocytes and tissue of rats with experimentally induced renal ischemia-reperfusion injury. The study included Wistar albino rats with a mean weight of 250 g. Study groups were formed as follows: control, sham-control, ischemia + reperfusion, zinc + ischemia-reperfusion, melatonin + ischemia-reperfusion, and zinc + melatonin + ischemia-reperfusion. Animals were supplemented with zinc and melatonin 3 mg/kg/day i.p. for 3 weeks before the induction of ischemia-reperfusion. Renal ischemia-reperfusion was induced in the left kidney under general anesthesia and consisted of ischemia for 45 minutes and reperfusion for 1 hour. After the procedure, animals were sacrificed and blood and kidney samples were collected to analyze MDA and GSH levels. GSH values in kidney tissues and erythrocytes were found to be elevated in the groups supplemented with zinc and melatonin (p<0.005). When MDA values in renal tissue and plasma were examined, it was seen that ischemia significantly elevated this parameter, while zinc and melatonin supplementation significantly inhibited MDA values (p<0.002). The results of the study indicated that oxidative injury of the blood and renal tissues of rats increased in association with ischemia-reperfusion, but zinc and melatonin supplementation before ischemia-reperfusion markedly reduced this oxidative damage.

Keywords

Acute kidney injury – prevention and control; Reperfusion injury – prevention and control; Zinc – therapeutic use; Melatonin – therapeutic use; Free radicals; Rats

Hrčak ID:

154804

URI

https://hrcak.srce.hr/154804

Publication date:

1.12.2015.

Article data in other languages: croatian

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