Original scientific paper
THE INFLUENCE OF ULTRAVIOLET LIGHT ON PATHOGENICITY OF ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA (BALSAMO) VUILLEMIN TO THE EUROPEAN CORN BORER, OSTRINIA NUBILALIS HBN. (LEPIDOPTERA: CRAMBIDAE)
Ľ. CAGÁŇ
M. ŠVERCEL
Abstract
The influence of different doses of ultraviolet (UV) light on the pathogenicity of entomopathogenic fungus
Beauveria bassiana (Balsamo) Vuillemin to the European corn borer, Ostrinia nubilalis Hbn., and radial
growth of fungus was studied in laboratory conditions. The suspensions of B. bassiana isolate SK99 were
exposed to UV light. Four different doses of UV light were used in the experiment. The distance between
exposed suspensions and UV light source was 0.3 m. Exposure duration was 15, 30, 45 and 60 minutes (as A,
B, C and D variants). Control variant SK99 and obtained variants SK99A, SK99B, SK99C and SK99D were
cultivated 21 days on Sabourard-dextrose agar. The larvae of O. nubilalis were infected with dry powder
consisted of mycelia and spores from fungus cultures. During 10 days, the mortality of infected larvae was
evaluated. It was ascertained that UV light exposition significantly influenced the mortality effect of B.
bassiana isolates to O. nubilalis larvae. Variant SK99C showed the highest level of infectivity. Radial growth
of UV variants was slower with rising time of exposure. The best ability to grow possessed non-irradiated
isolate SK99 and the worse variant SK99D. The difference between these two variants was significant.
Keywords
Beauveria bassiana; UV light; mutagenesis; Ostrinia nubilalis
Hrčak ID:
16021
URI
Publication date:
21.3.2002.
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