Original scientific paper
https://doi.org/10.15255/CABEQ.2015.2284
Physical and Biophysical Pretreatment of Water Hyacinth Biomass for Cellulase Enzyme Production
F. Amriani
orcid.org/0000-0003-2373-5651
; a)Indonesian Institute of Sciences, Research Center for Chemistry, Kawasan Puspiptek Serpong 15314, Indonesia; b)Department of Chemical Engineering University of North Sumatera Padang Bulan, Medan 20155, Indonesia
F. A. Salim
; Department of Chemical Engineering University of North Sumatera Padang Bulan, Medan 20155, Indonesia
I. Iskandinata
; Department of Chemical Engineering University of North Sumatera Padang Bulan, Medan 20155, Indonesia
D. Khumsupan
; The Petroleum and Petrochemical College, Center of Excellence for Petrochemical and Materials Technology, Chulalongkorn University, Bangkok 10330, Thailand
Z. Barta
; Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Budapest 1111, Hungary
Abstract
One practical way to control water hyacinth overgrowth due to eutrophication is by utilizing it as a substrate to produce cellulase. Water hyacinth was subjected to pretreatment to degrade lignin and improve microbes’ accessibility to cellulose. Physical and biophysical pretreatment methods were investigated. Biomass size reduction was performed in the physical pretreatment whereas white rot fungus (Ganoderma boninense) was used in the biophysical pretreatment. Cellulase-producing fungi, Aspergillus niger and Trichoderma reesei, were exploited in this study. Although lignin content was reduced
by two-fold after the biophysical pretreatment, the maximum production of cellulase occurred when only the physical pretreatment was employed on the substrate. It may be because the higher apparent crystallinity of cellulose in physical pretreatment triggers
more cellulase production compared to that in biophysical pretreatment. The maximum cellulase activity was found to be 1.035 IU mL–1 when water hyacinth was only physically pretreated.
Keywords
Aspergillus niger; cellulase; cellulose; pretreatment; Trichoderma reesei; water hyacinth
Hrčak ID:
161815
URI
Publication date:
14.7.2016.
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