Review article

https://doi.org/10.20471/acc.2016.55.02.15

Fibrin gel as a scaffold for skin substitute – production and clinical experience

Antun Kljenak ; Zagreb Children’s Hospital, Zagreb, Croatia
Mirna Tominac Trcin orcid.org/0000-0002-2918-7388 ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Marina Bujić ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Tamara Dolenec ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Martina Jevak ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Gordan Mršić ; Ivan Vučetić Center for Forensic Investigations, Research and Expertise, Zagreb, Croatia; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Gordana Zmiš ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Zoran Barčot orcid.org/0000-0001-7123-1429 ; Zagreb Children’s Hospital, Zagreb, Croatia
Ante Muljačić ; Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Maja Popović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of keratinocytes and fibroblasts on fibrin scaffold. Immunofluorescent staining of cell cultures on fibrin scaffold showed expression of vimentin, a marker of fibroblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of differentiated keratinocytes. Clinical results clearly showed that appearance of the skin did not differ significantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fibrin-cultured autografts on massive full-thickness burn resulted in good healing.

Keywords

Cells, cultured; Fibrin; Skin, artificial; Burns

Hrčak ID:

164870

URI

https://hrcak.srce.hr/164870

Publication date:

1.6.2016.

Article data in other languages: croatian

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