Original scientific paper
First report on PVL-positive methicillin-resistant Staphylococcus aureus of SCCmec type V, spa type t441 in Croatia
Ana Budimir
; Department of Clinical and Molecular Mirobiology, University Hospital Centre Zagreb, Croatia
Brigita Tićac
; Teaching Institute of Public Health, Department of Microbiology, School of Medicine University of Rijeka, Krešimirova ulica 52a, 51000 Rijeka, Croatia
Tomislav Rukavina
; Teaching Institute of Public Health, Department of Microbiology, School of Medicine University of Rijeka, Krešimirova ulica 52a, 51000 Rijeka, Croatia
Maja Farkaš
; Teaching Institute of Public Health, Department of Microbiology, Krešimirova ulica 52a, 51000 Rijeka, Croatia
Smilja Kalenić
; University of Zagreb, School of Medicine, Zagreb, Croatia
Abstract
Purpose
The aim of the study was to investigate the molecular epidemiology of community- associated MRSA in Primorsko-Goranska County of Croatia during a six-year period(2001-2007).
Methods
In period from 2001 and 2007, 46 MRSA isolates were collected in Rijeka, strains were subjected to susceptibility testing according to CLSI guidelines, mecA gene detection and SCCmec typing as well as detection of PVL. Strains were typed by Pulse Field Gel Electrophoresis (PFGE) and spa typing.
Results
All isolates were susceptible to vancomycin, linezolid, mupirocin, nitrofurantoin, only one strain was resistant to fusidic acid and co-trimoxazole.
Results of SCCmec typing showed the presence of SCCmec type IV in 26 MRSA strains, SCCmec type V in three strains, and 13 strains comprised SCCmec I. SCCmec type II and III were not observed.
Four MRSA strains were non-typeable by applied SCCmec typing methods.
PVL was detected in 4 strains, two SCCmec IV and two SCCmec V.
PFGE analysis, grouped MRSA strains into six similarity groups and 18 singletons.
Dominating spa types in this collection of strains were t015, with 15 strains, followed by t041(N=7), t051,(N=2 ), t2850(N=2), t008(N=2)and single isolates t441, t002, t448, t018, t019, t355, t390, t026, t449, t148. We also detected two new spa types, t3510 and t3509, respectively.
Conclusions
This is the first report on SCCmec type V in Croatia, and, to our knowledge, first report of PVL-positive mehicillin-resistant Staphylococcus aureus SCCmec type V and t441(ST59-MRSA-V) in this part of Europe.
Keywords
MRSA; Croatia; SCCmec; spa type
Hrčak ID:
166694
URI
Publication date:
28.6.2016.
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