Original scientific paper
https://doi.org/10.3325/cmj.2016.57.165
Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis, and immunotoxicity
Beata A. Zasońska
; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic, Prague, Czech Republic
Aurélia Líšková
; 2Medical Faculty, Slovak Medical University, Bratislava, Slovakia
Miroslava Kuricová
; 2Medical Faculty, Slovak Medical University, Bratislava, Slovakia
Jana Tulinská
; 2Medical Faculty, Slovak Medical University, Bratislava, Slovakia
Ognen Pop-Georgievski
; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic, Prague, Czech Republic
Fedor Čiampor
; Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia
Ivo Vávra
; Institute of Electrical Engineering Slovak Academy of Sciences, Bratislava, Slovakia
Mária Dušinská
; Health Effects Laboratory, Department of Environmental Chemistry, NILU-NorwegianInstitute for Air Research, Kjeller,Norway
Silvia Ilavská
; 2Medical Faculty, Slovak Medical University, Bratislava, Slovakia
Mira Horváthová
; 2Medical Faculty, Slovak Medical University, Bratislava, Slovakia
Daniel Horák
orcid.org/0000-0002-6907-9701
; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic, Prague, Czech Republic
Abstract
Aim To determine cytotoxicity and effect of silica-coated
magnetic nanoparticles (MNPs) on immune response, in
particular lymphocyte proliferative activity, phagocytic activity,
and leukocyte respiratory burst and in vitro production
of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma
(IFN-γ), tumor necrosis factor-alpha (TNF-α), and granulocyte
macrophage colony stimulating factor (GM-CSF).
Methods Maghemite was prepared by coprecipitation of
iron salts with ammonia, oxidation with NaOCl and modified
by tetramethyl orthosilicate and aminosilanes. Particles
were characterized by transmission electron microscopy
(TEM), dynamic light scattering (DLS), Fourier-transform infrared
(FTIR), and X-ray photoelectron spectroscopy (XPS).
Cytotoxicity and lymphocyte proliferative activity were
assessed using [3H]-thymidine incorporation into DNA of
proliferating human peripheral blood cells. Phagocytic activity
and leukocyte respiratory burst were measured by
flow cytometry; cytokine levels in cell supernatants were
determined by ELISA.
Results γ-Fe2O3&SiO2-NH2 MNPs were 13 nm in size. According
to TEM, they were localized in the cell cytoplasm
and extracellular space. Neither cytotoxic effect nor significant
differences in T-lymphocyte and T-dependent Bcell
proliferative response were found at particle concentrations
0.12-75 μg/cm2 after 24, 48, and 72 h incubation.
Significantly increased production of IL-6 and 8, and GMCSF
cytokines was observed in the cells treated with 3, 15,
and 75 μg of particles/cm2 for 48 h and stimulated with
pokeweed mitogen (PHA). No significant changes in TNF-α
and IFN-γ production were observed. MNPs did not affect
phagocytic activity of monocytes and granulocytes when
added to cells for 24 and 48 h. Phagocytic respiratory burst
was significantly enhanced in the cultures exposed to 75
μg MNPs/cm2 for 48 h.
Conclusions The cytotoxicity and in vitro immunotoxicity
were found to be minimal in the newly developed porous
core-shell γ-Fe2O3&SiO2-NH2 magnetic nanoparticles.
Keywords
Hrčak ID:
169496
URI
Publication date:
15.4.2016.
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