Original scientific paper
https://doi.org/10.3325/cmj.2016.57.180
Antioxidants selenomethionine and D-pantethine decrease the negative side effects of doxorubicin in NL/Ly lymphoma-bearing mice
Rostyslav R. Panchuk
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Nadia R. Skorokhyd
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Yuliya S. Kozak
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Liliya V. Lehka
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Vira V. Chumak
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Sofya N. Omelyanchik
; Laboratory of Metabolomics, Institute of Biochemistry of Biologically Active Compounds, Grodno, Belarus
Valery A. Gurinovich
; Laboratory of Metabolomics, Institute of Biochemistry of Biologically Active Compounds, Grodno, Belarus
Andrey G. Moiseenok
; Laboratory of Metabolomics, Institute of Biochemistry of Biologically Active Compounds, Grodno, Belarus
Rostyslav S. Stoika
; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
Abstract
Aim To investigate the potential tissue-protective effects
of antioxidants selenomethionine and D-pantethine applied
together with doxorubicin (Dx) on NK/Ly lymphomabearing
mice. The impact of this chemotherapy scheme
on animal survival, blood cell profile, hepatotoxicity, glutathione
level, and activity of glutathione-converting enzymes
in the liver was compared with the action of Dx applied
alone.
Methods The hematological profile of animals was studied
by the analysis of blood smears under light microscopy.
Hepatotoxicity of studied drugs was evaluated measuring
the activity of alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) enzymes, De Ritis ratio, and coenzyme
A fractions by McDougal assay. Glutathione level in
animal tissues was measured with Ellman reagent, and the
activity of glutathione reductase, transferase, and peroxidase
was measured using standard biochemical assays.
Results D-pantethine (500 mg/kg) and, to a lower extent,
selenomethionine (600 μg/kg) partially reduced the negative
side effects (leukocytopenia and erythropenia) of Dx (5
mg/kg) in NK/Ly lymphoma bearing animals on the 14th
day of their treatment. This increased animal survival time
from 47-48 to 60+ days and improved the quality of their
life. This ability of D-pantethine and selenomethionine was
realized via hepatoprotective and immunomodulating activities.
D-pantethine also restored the levels of acid-soluble
and free CoA in the liver of tumor-bearing animals,
while selenomethionine caused the recovery of glutathione
peroxidase levels in the liver, which was significantly
diminished under Dx treatment. Both compounds decreased
glutathione level in the liver, which was considerably
induced by Dx.
Conclusions Antioxidants selenomethionine and D-pantethine
partially reversed the negative side effects of Dx in
NK/Ly lymphoma-bearing mice and significantly increased
the therapeutic efficiency of this drug in tumor treatment.
Keywords
Hrčak ID:
169499
URI
Publication date:
15.4.2016.
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