Original scientific paper

https://doi.org/10.5562/cca2717

Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity

Lijun Yan ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China
Xiaofeng Wang ; Department of Precision Instruments, Tsinghua University, Beijing 100084, P. R. China
Qiutong Li ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China
Wencheng Wang ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China
Shixing Gong ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China
Zheng You ; Department of Precision Instruments, Tsinghua University, Beijing 100084, P. R. China
Peng Yi ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China
Wei Sun orcid.org/0000-0002-9923-7328 ; Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China

Abstract

In this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as the film forming material and the modified electrode was denoted as CTS/NiO-HRP/CILE. UV-Vis absorption and FT-IR spectra confirmed that HRP retained its native structure after mixed with NiO nanoflower. Direct electron transfer of HRP on the modified electrode was investigated by cyclic voltammetry with a pair of quasi-reversible redox waves appeared, indicating that the presence of NiO nanoflower on the electrode surface could accelerate the electron transfer rate between the electroactive center of HRP and the substrate electrode. Electrochemical behaviors of HRP on the modified electrode were carefully investigated. The HRP modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid with wider linear range and lower detection limit. Therefore the presence of NiO nanoflower could provide a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. The fabricated electrochemical biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

nickel oxide nanoflower; horseradish peroxidase; carbon ionic liquid electrode; direct electrochemistry; electrocatalysis

Hrčak ID:

170288

URI

https://hrcak.srce.hr/170288

Publication date:

28.9.2016.

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