Original scientific paper
https://doi.org/10.3325/cmj.2016.57.363
Cigarette smoke-induced chronic obstructive pulmonary disease is attenuated by CCL20-blocker: a rat model
Desheng Sun
; Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College,Huazhong University of Scienceand Technology, Wuhan, China
Yao Ouyang
; Department of Respiratory Medicine, Affiliated Hospital ofZunyi Medical College, Zunyi, China
Xiansheng Liu
; Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College,Huazhong University of Scienceand Technology, Wuhan, China
Abstract
Aim To evaluate whether the effect of dendritic cells (DCs)
on chronic obstructive pulmonary disease (COPD) can be
relieved by blocking CCL20.
Methods 30 Wistar rats were randomly divided into three
groups: control, COPD, and COPD treated with CCL20
monoclonal antibody. In the latter two groups, COPD was
induced by four-week cigarette smoke exposure and trachea
injection of lipopolysaccharide solution on two occasions.
CCL20 monoclonal antibody was injected intraperitoneally
on the first day. All animals were sacrificed on the
29th day. Pathomorphology of the lung and bronchiole
was analyzed using hematoxylin and eosin staining. The
CCR6 content in the bronchoalveolar lavage fluid was detected
using ELISA. DC distribution in the lung was examined
by immunohistochemistry for OX62.
Results COPD rat models showed pathological alterations
similar to those in COPD patients. DCs, CCR6, and the severity
of emphysema were significantly increased in the
COPD group than in controls (all P values <0.001), and
they were significantly reduced after anti-CCL20 treatment
compared with the COPD group (all P values <0.05).
Conclusion The interaction between CCR6 and its ligand
CCL20 promotes the effect of DCs in the COPD pathogenesis,
which can be reduced by blocking CCL20.
Keywords
Hrčak ID:
170340
URI
Publication date:
15.8.2016.
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