Original scientific paper

Thermal Perturbation of the Equilibrium System Ribonuclease A-Cytidine 3'-Monophosphate at pR 5.5

Mirna Flogel ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb
Tihana Žanić ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb

Abstract

The equilibrium system ribonuclease A-cytidine 3'-monophosphate
at pH 5.5, was submitted to thermal perturbation. A derivative
fractional degree of saturation with respect to a temperature raise of twenty degrees was recorded as a function of free ligand concentration. The analysis of the thermal perturbation curve directly yields L1HO and L1GO of the binding reaction. The evaluated thermodynamic parameters are in good agreement with the values obtained by other methods. The thermal perturbation technique proved to be an elegant, time and material saving experimental method in providing accurate thermodynamic information about ligand-macromolecule interactions.

Keywords

Hrčak ID:

177745

URI

https://hrcak.srce.hr/177745

Publication date:

22.5.1985.

Visits: 608 *