Review article

Possibilities of liquid biopsy in clinical practice

Josipa Periša orcid.org/0000-0002-1734-0015 ; Department of Medical Biochemistry in Oncology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Petra Bulić ; Department of Medical Biochemistry in Oncology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Zvjezdana Špacir Prskalo ; Department of Medical Biochemistry in Oncology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Mihela Gaće ; Department of Medical Biochemistry in Oncology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Ljiljana Mayer ; Department of Medical Biochemistry in Oncology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia

Abstract

Liquid biopsy represents innovation in the fi eld of oncology diagnosis. It refers to non-invasive blood collection method with isolation of circulating tumor cells, cell-free DNA (with circulating tumor cell free DNA fraction), circulating exosomes or micro RNA. Although requires specific methods for isolation and analysis of targets of interest, liquid biopsy could be of benefit in preoperative prediction od overall survival of patients with carcinoma, for monitoring of disease and progression, along with surveillance of therapy response and early recurrence of cancer. The review describes methods for liquid biopsy analysis, together with concerns and achievements in implementing liquid biopsy in clinical practice.

Keywords

liquid biopsy; circulating tumor DNA - ctDNA; circulating tumor cells – CTCs; circulating microRNA – cmiRNA; exosomes; clinical practice

Hrčak ID:

184608

URI

https://hrcak.srce.hr/184608

Publication date:

3.7.2017.

Article data in other languages: croatian

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