Original scientific paper

How Surface Heterogeneity Affects Protein Adsorption: Annealing of OTS Patterns and Albumin Adsorption Kinetics

Gerald N. Hodgkinson ; Department of Bioengineering, University of Utah, Salt Lake City, UT, USA
Vladimir Hlady ; Department of Bioengineering, University of Utah, Salt Lake City, UT, USA

Abstract

Fluorescence microscopy and intensity histogram analysis techniques were used to monitor
spatially-resolved albumin adsorption kinetics to model heterogeneous surfaces on sub-mm
scales. Several distinct protein subpopulations were resolved, each represented by a normal
distribution of adsorption densities on the adsorbent surface. Histogram analyses provided
dynamic information of mean adsorption density, spread in adsorption density, and surface area
coverage for each distinct protein subpopulation. A simple adsorption model is proposed in
which individual protein binding events are predicted by the summation of multiple protein’s
surface sub-site interactions with different binding energy sub-sites on adsorbent surfaces. This
model is predictive of the albumin adsorption on the patterns produced by one step m-contact
printing (mCP) of octadecyltrichlorosilane (OTS) on glass but fails to describe adsorption once
the same patterns are altered by a thermal annealing step.

Keywords

adsorption kinetics; albumin; surface heterogeneity; fluorescence imaging

Hrčak ID:

18664

URI

https://hrcak.srce.hr/18664

Publication date:

30.11.2007.

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