Original scientific paper

https://doi.org/10.11613/BM.2018.010702

Fasting conditions: Influence of water intake on clinical chemistry analytes

Silvia F. Benozzi ; Bioquímica Clínica I, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina
Gisela Unger ; Bioquímica Clínica I, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina
Amparo Campion ; Bioquímica Clínica I, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina; Hospital Municipal de Agudos ‘‘Dr. Leónidas Lucero’’, Bahía Blanca, Argentina
Graciela L. Pennacchiotti ; Bioquímica Clínica I, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina; Hospital Municipal de Agudos ‘‘Dr. Leónidas Lucero’’, Bahía Blanca, Argentina

Abstract

Introduction: Currently available recommendations regarding fasting requirements before phlebotomy do not specify any maximum water intake
volume permitted during the fasting period. The aim was to study the effects of 300 mL water intake 1 h before phlebotomy on specific analytes.
Materials and methods: Blood was collected from 20 women (median age (min-max): 24 (22 - 50) years) in basal state (T0) and 1 h after 300 mL
water intake (T1). Glucose, total proteins (TP), urea, creatinine, cystatin C, total bilirubin (BT), total cholesterol, high-density lipoprotein cholesterol,
low-density lipoprotein cholesterol, triglycerides (Tg), uric acid (UA), high-sensitivity C-reactive protein, gamma-glutamyl transferase (GGT),
aspartate-aminotransferase (AST), alanine-aminotransferase and lactate-dehydrogenase (LD) were studied. Results were analyzed using Wilcoxon
test. Mean difference (%) was calculated for each analyte and was further compared with reference change value (RCV). Only mean differences (%)
higher than RCV were considered clinically significant.
Results: Significant differences (median T0 vs median T1, P) were observed for TP (73 vs 74 g/L, 0.001); urea (4.08 vs 4.16 mmol/L, 0.010); BT (12
vs 13 μmol/L, 0.021); total cholesterol (4.9 vs 4.9 mmol/L, 0.042); Tg (1.05 vs 1.06 mmol/L, 0.002); UA (260 vs 270 μmol/L, 0.006); GGT (12 vs 12
U/L, 0.046); AST (22 vs 24 U/L, 0.001); and LD (364 vs 386 U/L, 0.001). Although the differences observed were statistically significant, they were not
indicative of clinically significant changes.
Conclusions: A water intake of 300 mL 1 h prior to phlebotomy does not interfere with the analytes studied in the present work.

Keywords

fasting; water intake; blood sample collection; preanalytical variability; clinical chemistry tests

Hrčak ID:

189592

URI

https://hrcak.srce.hr/189592

Publication date:

15.2.2018.

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