Original scientific paper

https://doi.org/10.11613/BM.2018.010704

Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new BD-Barricor tubes

Janne Cadamuro ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria
Cornelia Mrazek ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria
Alexander B. Leichtle ; University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Switzerland
Ulrike Kipman ; UT SPSS Statistics, Hallein, Austria
Thomas K. Felder ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria
Helmut Wiedemann ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria
Hannes Oberkofler ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria
Georg M. Fiedler ; University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Switzerland
Elisabeth Haschke-Becher ; Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria

Abstract

Introduction: Although centrifugation is performed in almost every blood sample, recommendations on duration and g-force are heterogeneous
and mostly based on expert opinions. In order to unify this step in a fully automated laboratory, we aimed to evaluate different centrifugation
settings and their influence on the results of routine clinical chemistry analytes.
Materials and methods: We collected blood from 41 healthy volunteers into BD Vacutainer PST II-heparin-gel- (LiHepGel), BD Vacutainer SST IIserum-,
and BD Vacutainer Barricor heparin-tubes with a mechanical separator (LiHepBar). Tubes were centrifuged at 2000xg for 10 minutes and
3000xg for 7 and 5 minutes, respectively. Subsequently 60 and 21 clinical chemistry analytes were measured in plasma and serum samples, respectively,
using a Roche COBAS instrument.
Results: High sensitive Troponin T, pregnancy-associated plasma protein A, ß human chorionic gonadotropin and rheumatoid factor had to be
excluded from statistical evaluation as many of the respective results were below the measuring range. Except of free haemoglobin (fHb) measurements,
no analyte result was altered by the use of shorter centrifugation times at higher g-forces. Comparing LiHepBar to LiHepGel tubes at different
centrifugation setting, we found higher lactate-dehydrogenase (LD) (P = 0.003 to < 0.001) and lower bicarbonate values (P = 0.049 to 0.008) in the
latter.
Conclusions: Serum and heparin samples may be centrifuged at higher speed (3000xg) for a shorter amount of time (5 minutes) without alteration
of the analytes tested in this study. When using LiHepBar tubes for blood collection, a separate LD reference value might be needed.

Keywords

pre-analytics; centrifugation; diagnostic tests; routine

Hrčak ID:

189594

URI

https://hrcak.srce.hr/189594

Publication date:

15.2.2018.

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