Short communication, Note

https://doi.org/10.2478/acph-2018-0002

HPTLC determination of diosgenin in fenugreek seeds

BARBARA KRÓL-KOGUS ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, Poland
KHENIFI MOHAMMED LAMINE ; Laboratoire d’Ethnobotanique et Substances Naturelles, Ecole Normale Supérieure de Kouba, B.P 92 Kouba, 16050 Algerie
PIOTR MIGAS ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, Poland
MESSAOUD BOUDJENIBA ; Laboratoire d’Ethnobotanique et Substances Naturelles, Ecole Normale Supérieure de Kouba, B.P 92 Kouba, 16050 Algerie
MIROSŁAWA KRAUZE-BARANOWSKA ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, Poland

Abstract

A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L–1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates using a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12–0.18 %.

Keywords

fenugreek (Trigonella foenum-graecum); diosgenin; HPTLC; quantitative analysis

Hrčak ID:

189690

URI

https://hrcak.srce.hr/189690

Publication date:

31.3.2018.

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