Short communication, Note

https://doi.org/10.2478/acph-2018-0009

Chronic fluoxetine treatment induces lipid accumulation but does not alter the expression of Pref-1 in adipose tissue of rats

LÁSZLÓ-ISTVÁN BÁBA orcid.org/0000-0001-8682-0429 ; County Hospital of Mures, Tîrgu Mures, Romania
ZSOLT GÁLL orcid.org/0000-0002-9091-277X ; Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania
ISTVÁN LÓRÁNT BÍRÓ ; County Hospital of Mures, Tîrgu Mures, Romania
TIBOR MEZEI orcid.org/0000-0001-5150-7993 ; Department of Pathology, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania
IMRE ZOLTÁN KUN ; Doctoral School, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania
MELINDA KOLCSÁR ; Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania

Abstract

This study aims to investigate the effects of chronic fluoxetine (FLX) treatment on preadipocyte factor-1 (Pref-1) expression in subcutaneous, visceral and brown adipose tissues, and on the size of vacuoles in adipocytes obtained from the perirenal regions in rats. Twenty-eight Wistar rats were treated with FLX at two different doses and fourteen animals received vehicle. After 40 days of treatment, the subcutaneous, perirenal and interscapular adipose tissues were collected. Pref-1 expression was examined using an immunohistochemical method and the vacuolar area was measured in stained sections. In the low dose FLX group, the size of vacuoles increased both in male and female animals. The high dose of FLX also induced a significant increase of vacuole size, but only in male animals. Neither of the two doses of FLX has significantly affected the Pref-1 expression in any type of adipose tissue.

Keywords

fluoxetine; adipose tissue; preadipocyte factor-1; vacuole size

Hrčak ID:

190215

URI

https://hrcak.srce.hr/190215

Publication date:

31.3.2018.

Visits: 1.317 *