Original scientific paper

Anticathepsin D Antibody-Sepharose Chromatography of Human Cathepsin D

J. Babnik ; Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia
T . Lah ; Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia
V. Cotič ; Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia
V. Turk ; Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia

Abstract

Cathepsin D was isoilated from human tissues by anticatheipsin
D antibo:dy-Serpharose 4B .chromatoigraphy. Caithepsin D,
rele1ased from the immunoaffinity column formed one precipitin
line with specif.ic antibody in immwnod:Lffusion and in immunoe1ectropholl1esis.
The isolated proteinase is shown to be prure
cathepsin D by actiivity and by inhibition with peipstatin.
The quanititative determinati.on o:f cathepsin D in human
tissue, taiken by bio.psy from sco,Uotic patients 1rl ivery small
amount, is desert.bed.
It was demonstrated that cathepsin D from human muscle
and human gingival fluid was indistinguishable in immunodiffus
ion from the human liver catheps iirl D.

Keywords

Hrčak ID:

194156

URI

https://hrcak.srce.hr/194156

Publication date:

15.9.1984.

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