Original scientific paper

Crystalline Suspension of Bovine Zinc Des-AlaB30-Insulin with Prolonged Hypoglycaemic Activity

Biserka Mulac-Jeričević ; Tracer Laboratory, Department of Organic Chemistry and Biochemistry »Rueter Boiikovic« Institute, POB 1016, 41001 Zagreb,
Branka Vranešić ; Research Department PLIVA, Zagreb, Yugoslavia
Sergije Kveder ; Tracer Laboratory, Department of Organic Chemistry and Biochemistry »Rueter Boiikovic« Institute, POB 1016, 41001 Zagreb,
Blanka Jamnicky ; Research Department PLIVA, Zagreb, Yugoslavia
Dina Keglević ; Tracer Laboratory, Department of Organic Chemistry and Biochemistry »Rueter Boiikovic« Institute, POB 1016, 41001 Zagreb,

Abstract

The reversible reaction of cyclohexane-1,2-dione with the
guanidino function of Arg-B22 in bovine insulin gave the dihydroxycyclohexylene
[DHCH]-Arg-B22-insulin complex (I) which,
after selective tryptic cleavage at the Lys-B29 residue and regeneration
of the guanidino group, afforded bovine des-AlaB30-
-insulin (III) in an overal 85.80/o yield. The truncated insulin III
was converted into the regular zinc salt and, under conditions
that favour binding of more than 2 Zn atoms per insulin hexamer
unit, into a crystalline suspension. When tested for their hypoglycaemic
activity, the two forms of III exhibited significantly different
durations of the effect in mice: the blood glucose curves
obtained for the III-Zn salt and the III-crystalline suspension
were indistinguishable from those exerted by regular- and long-
acting bovine zinc insulin, respectively.

Keywords

Hrčak ID:

194165

URI

https://hrcak.srce.hr/194165

Publication date:

6.4.1984.

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