Original scientific paper
High sensitive troponin concentration stability in dialysate of anuric patients on hemodialysis
INGRID PRKAČIN
orcid.org/0000-0002-5830-7131
; Clinical Hospital Merkur, I.Zajca 19, Zagreb
ŽELJKA JUREKOVIĆ
SONJA PERKOV
ANA SAVUK
TAMARA PIKIVACA
ANTONIO GOLUB
TOMO SVAGUŠA
orcid.org/0000-0002-2036-1239
Abstract
Background. High sensitive troponin I
(hsTnI) and high sensitive troponin T
(hsTnT) are markers of cardiac damage.
Cardiomyocyte necrosis increases its
blood levels. It is known that dialysis is
cardiotoxic and that results in lack of contractility
of certain myocardial segments.
Tis mechanism is primarily due to hypo
perfusion of the myocardium during dialysis.
Te dialysis itself increases cardiovascular
(CV) risk in patients by many different
mechanisms. It has been proven that
the incidence of heart failure is much more
frequent in patients on hemodialysis than
in healthy population.
Te aim of this pilot study was to investigate
the presence of troponin T molecules
and troponin I in dialysate and compare
their concentrations.
Materials and Methods. Te study included
5 anuric patients (4M) on hemodialysis.
Te dialysate samples were sampled for
each patient three times during a dialysis
cycle. Te frst sample was taken afer thirty
minutes, the second sample was taken
in the middle of dialysis (120 minutes) and
the third sample was taken thirty minutes
before the end of dialysis. Te value of hsTnI
was measured using a high-sensitivity
test on the Immuno-enzymatic analyzer
Abbott Architest i1000SR. According to
CLSI EP15-A2 protocol verifcation of
hsTnT chemiluminescent micro-particle
immunoassay on the analytical platform
Roche cobas e411 was performed.
Results. Altogether 15 samples (three for
each patient) were processed. hsTnT was
detected in all 15 samples (13.42 ± 1.18
ng / L), while hsTnI was detected in only 8
samples (0.14 ± 0.16 ng / L). To test the difference
in detectability between hsTnT and
hsTnI, chi square test was used and the difference
was statistically signifcant (Yates
chi-square 6.708, p = 0.009).
Conclusion. Te presence of troponin
molecules in dialysate was determined for
the frst time in scientifc literature. Tis
study has confrmed that TnT is present in
all dialysate samples and that its concentration
is stable in dialysate. TnI concentrations
were detectable in signifcantly lower
concentrations.
Keywords
hemodialysis; hs troponin T; hs troponin I; dialysate
Hrčak ID:
195361
URI
Publication date:
1.3.2018.
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