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Croatica Chemica Acta, Vol. 52 No. 4, 1979.

Original scientific paper

Isolation of Cathepsin Band a-N-Benzoylarginine-B-naphthylamide Hydrolase by Covalent Chromatography on Activated Thiol Sepharose

T. Zvonar ; Department of Biochemistry, J. Stefan Institute, 61000 Ljubljana
I. Kregar ; Department of Biochemistry, J. Stefan Institute, 61000 Ljubljana
V. Turk ; Department of Biochemistry, J. Stefan Institute, 61000 Ljubljana

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Abstract

Cathepsin Band a-N-benzoylarginine-P,-naphthylamide (BANA)
hydrolase have been isolated from bovine lymph nodes using a
novel procedure that includes besides gel filtration and ion exchange
chromatography also covalent chromatography as the
essential step. Both enzymes were selectively bound to the
activated thiol-Sepharose and afterwards eluted with cysteine. The
homogeneity of enzymes was proved by polyacrylamide gel electrophoresis.

Keywords

Hrčak ID:

195863

URI

https://hrcak.srce.hr/195863

Publication date:

1.3.1980.

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