Original scientific paper

Proton Magnetic Relaxation in Ethane Diol-Water Solutions of Haemoglobin

J. Brnjas-Kraljević ; Institute of Biology and Institute of Physics, University of Zagreb, 41000 Zagreb, Croatia, Yugoslavia
S. Maričić ; Institute of Biology and Institute of Physics, University of Zagreb, 41000 Zagreb, Croatia, Yugoslavia
S . Vuk-Pavlović ; Institute of Biology and Institute of Physics, University of Zagreb, 41000 Zagreb, Croatia, Yugoslavia

Abstract

Ethane diol was added to aqueous solutions of bovine and
human met(FeUI)- and CO-haemoglobin in order to extend the
temperature range for nuclear magnetict relaxation measurements
below freezing point. No significant difference in the relaxation
rates was found on adding ethane diol except in the case of human
met- and CO-haemog1obin, which may be . ascribed to chang·es
in the hydration sheath. The energies of activation derived :firom
Arrhenius plots of the relaxation rates for bovine Hb are also
independent (within ± 50/o) of ethane diol. It is concluded that the
gross conformation of the haem pocket is not altered by addition
of ethane diol, so that measurements can be done down to - 30 °c.

Keywords

Hrčak ID:

197012

URI

https://hrcak.srce.hr/197012

Publication date:

15.4.1974.

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