Original scientific paper

A Photometric Method for the Determination of Serum Titres by Latex Particle Agglutination

N. Deželić ; Laboratory of Biocolloidal Chemistry, Andrija . Stampar School of Public Health, Faculty of Medicine, University of Zagreb, Zagreb, Croatia, Yugoslavia
Gj. Deželić ; Laboratory of Biocolloidal Chemistry, Andrija . Stampar School of Public Health, Faculty of Medicine, University of Zagreb, Zagreb, Croatia, Yugoslavia

Abstract

A simple photometric method for the determination of latex
particle agglutination in immunochemical systems is described
This photometric latex test (PLT) allows the determination of
serum titres by the numerical evaluation of measured turbidities.
A mathematical algorithm for data processing by a desk-top
computer is desfgned. From the experiments with a model
immunochemical system (human serum albumin - rabbit antiserum)
it was found that with the PLT the reliability and reproducibility
in the serum titre determination was significantly
increased in comparison to standard latex-fixation tests with the
visual detection of agglutination. It was found that the PLT is
at '"least as sensitive as passive hemagglutination tests. The PLT
can easily be applied in routine immunochemical and serologic
work and represents an improvement of existing quantitative
latex agglutination tests.

Keywords

Hrčak ID:

197536

URI

https://hrcak.srce.hr/197536

Publication date:

30.10.1970.

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