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Croatica Chemica Acta, Vol. 90 No. 4, 2017.

Original scientific paper

https://doi.org/10.5562/cca3309

Mannosylated Liposomes with Built-in Peptidoglycan Based Immunomodulators for Subunit Vaccine Formulations

Adela Štimac ; University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia
Krešo Bendelja ; University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia
Maja Dutour Sikirić ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000, Zagreb, Croatia
Leo Frkanec ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000, Zagreb, Croatia
Ruža Frkanec ; University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia

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Abstract

The aim of the present study is preparation of mannosylated liposomes with built-in small molecule immunopotentiators for targeted, receptors mediated, delivery of antigens. The liposomes were mannosylated in two ways, by covalent attachment of p-aminophenyl-α-D-mannopyranoside to the preformed liposomes and by incorporation of synthetic mono-, di- and tetramannosyl-lipoconjugates into the lipid bilayer of liposomes. Four different mannosylated liposome formulations with incorporated model antigen, ovalbumin (OVA), and immunomodulators, PGM and Ad2TP2, were prepared and characterized. The influence of mannosylated liposome formulations on the antigen-specific humoral immune response was investigated. It has been shown that mannosylated liposomal formulations did not enhance the humoral immune response and production of anti-OVA antibodies but they significantly affected the type of OVA specific immune reaction and directed it towards Th1 type.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

mannosylated liposomes; targeted delivery; peptidoglycan monomer; adamantyltripeptide; adjuvants; subunit vaccines

Hrčak ID:

197960

URI

https://hrcak.srce.hr/197960

Publication date:

29.12.2017.

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