Original scientific paper

https://doi.org/10.5562/cca3298

Chemical Composition, Antioxidant and Antibacterial Activity of Different Extracts of Poplar Type Propolis

Tomislav Mašek ; Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR-10000 Zagreb, Croatia
Nataša Perin orcid.org/0000-0002-3900-4514 ; Department of Organic Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia
Livio Racané ; Department of Applied Chemistry, Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovića 28a, HR-10000 Zagreb, Croatia
Maja Cindrić orcid.org/0000-0002-3066-5631 ; Department of Organic Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia
Hana Čipčić Paljetak ; Division of Molecular Medicine, Laboratory for Epigenomics, School of Medicine, University of Zagreb, Šalata 2, HR-10000 Zagreb, Croatia
Mihaela Perić ; Division of Molecular Medicine, Laboratory for Epigenomics, School of Medicine, University of Zagreb, Šalata 2, HR-10000 Zagreb, Croatia
Mario Matijašić orcid.org/0000-0001-9209-4611 ; Division of Molecular Medicine, Laboratory for Epigenomics, School of Medicine, University of Zagreb, Šalata 2, HR-10000 Zagreb, Croatia
Donatella Verbanac orcid.org/0000-0002-9106-1604 ; Division of Molecular Medicine, Laboratory for Epigenomics, School of Medicine, University of Zagreb, Šalata 2, HR-10000 Zagreb, Croatia
Božo Radić ; Hedera, d.o.o, 4. gardijske brigade 35, Kamen HR-21000 Split, Croatia
Jelena Šuran ; Department of Pharmacology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR-10000 Zagreb, Croatia
Kristina Starčević orcid.org/0000-0002-2606-7807 ; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR-10000 Zagreb, Croatia

Abstract

Chemical composition, antioxidant activity and in vitro antibacterial activity of propolis from the central, continental part of Croatia were studied. Propolis hydro-ethanolic extracts (PHEE), prepared using three different methods and two solvent mixtures contained high amounts of flavonoids (20.95–28.11 % TIC), aromatic acids (8.17–15.91 % TIC) and their esters (9.27-11.91 % TIC). The PHEE obtained in this study showed high antioxidant activity (DPPH IC50 values from 9.96–19.95 µg/ml and FRAP 38.0–41.9 mM Fe2+/mg PHEE). Despite differences in composition, the PHEE samples exhibited significant antibacterial activities, affecting tested strains of Staphylococcus aures, Enterococcus faecalis, Escherichia coli and Moraxella catarrhalis. The use of different solvent ratio and extraction procedures selectively increases or decreases the content of specific propolis components in the extract which can have a beneficial effect on the application of propolis extracts.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

Propolis; Antioxidant activity; Antibacterial agents

Hrčak ID:

199968

URI

https://hrcak.srce.hr/199968

Publication date:

2.1.2018.

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