Original scientific paper

https://doi.org/10.24099/vet.arhiv.170110

Antimicrobial resistance of Escherichia coli isolated from healthy dogs in Lithuania

Jūratė Šiugždaitė ; Department of Veterinary Pathobiology, Lithuanian University of Health Science, Veterinary Academy, Kaunas, Lithuania
Aušra Gabinaitienė ; Department of Veterinary Pathobiology, Lithuanian University of Health Science, Veterinary Academy, Kaunas, Lithuania
Sigita Kerzienė ; Department of Social Sciences, Lithuanian University of Health Science, Veterinary Academy, Kaunas, Lithuania
Saulius A. Laurusevičius ; Private Veterinary Clinic, Kaunas, Lithuania
Tomas Laurusevičius ; Private Veterinary Clinic, Kaunas, Lithuania

Abstract

The objective of this study was to establish the prevalence and antimicrobial resistant patterns of commensal Escherichia coli isolated from healthy dogs. In total, 55 rectal swabs from clinically healthy dogs, kept outside (n = 20), and inside a house (n = 20) and from dogs kept at an animal shelter (n = 15), were collected for isolation of Escherichia coli. Resistance patterns to 11 antimicrobial agents were tested using E-test (Epsilometer test) to determine the MIC (Minimum Inhibitory Concentration). Multiplex polymerase chain reaction (M-PCR) amplification was used to detect selected genes conferring resistance to beta-lactams, tetracycline, aminoglycoside, sulphanilamide, quinolone, and phenicol classes of antimicrobial agents. Forty-eight E. coli strains were isolated from 55 (87.3%) dogs. Multi-drug resistance was present in 38% of resistant isolates. E. coli isolates showed the highest resistance rates to streptomycin (85.1%), ampicillin (77.1%), sulfamethoxazole (70.8%) and tetracycline (64.6%). The isolates were most sensitive to enrofloxacin (87.5%) and chloramphenicol (72.9%). Bacterial resistance genes were determined to tetracycline (tet) (9.7%) trimethoprim/sulfamethoxazole (dfrA1) (16.7%,), and chloramphenicol (catA1) (5.5%). In general, the prevalence of antimicrobial resistance in E. coli isolates from shelter dogs population was higher than in those from dogs kept inside and outside (P<0.05). Companion animals in Lithuania are important reservoirs of resistant Escherichia coli strains. Only appropriate use of antimicrobials can minimize the spread of resistant bacteria among healthy and diseased animals and humans.

Keywords

antimicrobial resistance; commensal E. coli; healthy dogs

Hrčak ID:

202682

URI

https://hrcak.srce.hr/202682

Publication date:

4.6.2018.

Article data in other languages: croatian

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