Croatica Chemica Acta, Vol. 91 No. 2, 2018.
Review article
https://doi.org/10.5562/cca3357
Identification of Stable and Enantioselective Lipases for Biotechnological Applications
Karl-Erich Jaeger
; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany
Alexander Fulton
; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany
Ulrich Schwaneberg
; RWTH Aachen University, Lehrstuhl für Biotechnologie, Worringer Weg 3, D-52074, Aachen, Germany
Jörg Pietruszka
; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, D-52426 Jülich, Germany
Filip Kovacic
orcid.org/0000-0002-0313-427X
; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany
Abstract
The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number of protein variants. Additionally, novel enzymes can be identified from metagenomic libraries, which contain bacterial clones expressing DNA isolated from different habitats. Activity-based screening of such libraries is needed to identify enzyme candidates of interest. Here, we provide a protocol for screening of regio- and enantioselective lipases stable under industrially relevant conditions.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
lipases; screening; biotechnology; stability; enantioselectivity; enzyme assays; high throughput
Hrčak ID:
203538
URI
Publication date:
4.6.2018.
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