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Acta Pharmaceutica, Vol. 68 No. 4, 2018.

Original scientific paper

https://doi.org/10.2478/acph-2018-0023

In vitro investigation of potential anti-diabetic activity of the corm extract of Hypoxis argentea Harv. Ex Baker

AKINLEYE AKINRINDE orcid id orcid.org/0000-0001-6883-4595 ; Plant stress response group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice, 5700, South Africa
TREVOR KOEKEMOER ; Medicinal Plants & Natural Products Research Group, Department of Biochemistry and Microbiology Nelson Mandela Metropolitan University, Port Elizabeth, South Africa
MARYNA VAN DE VENTER orcid id orcid.org/0000-0003-3816-1419 ; Medicinal Plants & Natural Products Research Group, Department of Biochemistry and Microbiology Nelson Mandela Metropolitan University, Port Elizabeth, South Africa
GRAEME BRADLEY orcid id orcid.org/0000-0002-9036-9869 ; Plant stress response group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice, 5700, South Africa

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Abstract

The corms of Hypoxis argentea are widely used as a traditional remedy for diabetes mellitus in South Africa. In this study, we investigated the effects of non-toxic concentrations (12.5–100 µg mL–1) of the aqueous extract of H. argentea (HAA) corms on glucose uptake, pancreatic beta cell proliferation, and adipocyte differentiation. HAA stimulated glucose uptake in HepG2 cells up to 19.6 % and 17.0 % in L6 myotubes. Live-cell imaging microscopy revealed significant increases (p < 0.001) in total INS-1 cell numbers exposed to HAA, although no effect was observed on adipogenesis in 3T3-L1 pre-adipocytes. HAA produced weak to moderate inhibition of porcine pancreatic α-amylase, α-glucosidase, porcine pancreatic lipase, dipeptidyl peptidase IV (DPP IV) activities, as well as protein glycation. Our results suggest that the acclaimed anti-diabetic effects of H. argentea could be mediated by its promotion of glucose utilization and preservation of pancreatic beta cell populations while preventing fat accumulation in adipocytes.

Keywords

Hypoxis argentea; diabetes; HepG2; INS-1; 3T3-L1; L6 myotubes; glucose uptake; adipogenesis

Hrčak ID:

205796

URI

https://hrcak.srce.hr/205796

Publication date:

31.12.2018.

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