Original scientific paper

https://doi.org/10.18047/poljo.24.2.4

Efficient micropropagation and rooting of Micromeria croatica (Pers.) Schott (Lamiaceae)

Snježana Kereša orcid.org/0000-0003-4034-568X ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Zoe Andrijanić orcid.org/0000-0001-7139-1852 ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Dario Kremer orcid.org/0000-0002-4050-4575 ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, Croatia
Ivanka Habuš Jerčić orcid.org/0000-0002-2962-9043 ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Marijana Barić ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Kristina Batelja Lodeta orcid.org/0000-0002-8488-3490 ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Snježana Bolarić orcid.org/0000-0002-0555-8922 ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Anita Bošnjak Mihovilović ; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia

Abstract

Micromeria croatica is an endangered species with a great potential for use as a medical as well as ornamental plant. For commercial use, an efficient propagation protocol is required. The aims of this study were to establish an efficient protocol for micropropagation and rooting of M. croatica. A number of factors were tested including the influence of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) at different concentrations as well as the effect of explant orientation and topophysis on shoot proliferation. Rooting was investigated in agar-based medium supplemented with indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at various concentrations. Media with 1 and 2 mg/L of BAP produced the highest number of shoots/explant. However, the lower concentration of BAP should be recommended due to longer shoots without any sign of hyperhydration. Explant orientation and node position showed equal capability for shoot proliferation. Importantly, 100% efficiency of rooting was achieved in an agar-based rooting medium. Acclimatisation exceeded 90%. Taken together, an efficient protocol for micropropagation and rooting of M. croatica has been established for future production.

Keywords

in vitro propagation; Micromeria croatica; plant growth regulators; topophysis

Hrčak ID:

214177

URI

https://hrcak.srce.hr/214177

Publication date:

19.12.2018.

Article data in other languages: croatian

Visits: 2.217 *