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Original scientific paper

https://doi.org/10.5562/cca3444

Electrochemical Determination of Metallothioneins by the Modified Brdička Procedure as an Analytical Tool in Biomonitoring Studies

Tatjana Mijošek ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Marijana Erk ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Vlatka Filipović Marijić ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Nesrete Krasnići ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Zrinka Dragun ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Dušica Ivanković ; Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička cesta 54, HR-10000 Zagreb, Croatia


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Abstract

Present study aims to improve the modified Brdička procedure of metallothionein (MT) quantification in a way to reduce the analysis duration. Therefore, scan rate of the voltammetric sweep was increased from 0.0052 V s–1 to 0.013 V s–1 by lowering the duration of one step potential from 0.5 s to 0.2 s, which resulted in 2.5 times faster voltammetric sweep. Research confirmed high accuracy and reliability of MT measurements, in both standard sample and samples of heat-treated cytosols of liver, gills and the intestine of European chub and liver of brown trout. The method was confirmed as fast and reliable electrochemical technique for quantification of MTs and this time-saving improvement is especially useful and applicable in different biomonitoring studies which require the analyses of numerous biological samples and high sample throughput.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

metallothioneins, differential pulse voltammetry, modified scan rate, fish tissues, biomarkers, biomonitoring

Hrčak ID:

217352

URI

https://hrcak.srce.hr/217352

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