Original scientific paper

https://doi.org/10.2478/acph-2020-0037

Phenolic compounds of Iris adriatica and their antimycobacterial effects

SANDRA OCHENSBERGER ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010 Graz, Austria
FABIAN ALPERTH ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010 Graz, Austria
BOŽENA MITIĆ ; University of Zagreb, Faculty of Science, Department of Biology (Botany), Marulicev trg 9a, HR-10000 Zagreb, Croatia
OLAF KUNERT ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Universitätsplatz 1, 8010 Graz, Austria
STEFANIE MAYER ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010 Graz, Austria
MARIA FERREIRA MOURÃO ; University of Lisbon, Faculty of Pharmacy, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal
IVANA TUREK ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010 Graz, Austria
SIMON VLAD LUCA ; Department of Pharmacognosy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii 16, 700115 Iasi, Romania
KRYSTYNA SKALICKA-WOŹNIAK ; Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland
ŽELJAN MALEŠ ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, HR-10000 Zagreb, Croatia
DARIO HRUŠEVAR ; University of Zagreb, Faculty of Science, Department of Biology (Botany), Marulicev trg 9a, HR-10000 Zagreb, Croatia
IVAN DUKA ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, HR-10000 Zagreb, Croatia
FRANZ BUCAR orcid.org/0000-0002-7417-616X ; University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010 Graz, Austria

Abstract

Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant en-demic to Dalmatia (Croatia). For this purpose, we performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc² 155. One obtained fraction was found to be antimyco-bacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly the benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone to be responsible for the resistance-modulatory activity of this plant.

Keywords

Iris adriatica; Radix Iridis; phenolic compounds; Mycobacterium smegmatis; re-sistance modulation; ethidium bromide; MIC

Hrčak ID:

220899

URI

https://hrcak.srce.hr/220899

Publication date:

31.12.2019.

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