Original scientific paper

https://doi.org/10.20471/acc.2019.58.02.18

Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer

Mateusz Rubinkiewicz ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland
Izabela Siemińska ; Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, Kraków, Poland
Piotr Małczak ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Piotr Major ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Jarosław Baran ; Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, Kraków, Poland
Andrzej Budzyński ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Michał Pędziwiatr orcid.org/0000-0001-7087-6639 ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland

Abstract

Surgical procedure has immense impact on the immune balance. However, little is known about perioperative changes in T regulatory and Th17 lymphocyte subpopulations in patients undergoing colorectal resection. Patients with resectable colon cancer were enrolled in the study. Blood samples were obtained on two occasions, i.e. before the procedure and two days after the surgery. We also recruited a control group of young, healthy individuals. Lymphocyte subpopulations were analyzed with the use of flow cytometry. Investigated subpopulations consisted of total lymphocyte count, CD4+, CD8+, T regulatory Foxp3+ (Tregs), Th17 lymphocytes and white blood cell (WBC) count. There were significant differences in immune cell levels before and after the surgery. Reduction was recorded in the CD4+, CD8+, Tregs and total lymphocyte counts (p=0.002, p=0.01, p=0.008 and p=0.001, respectively). Increase was observed in total WBC and Th17 cells, however, Th17 lymphocytes did not reach statistical significance (p=0.01 and p=0.5, respectively). In conclusion, surgical intervention caused changes in all lymphocyte subpopulations investigated in patients undergoing surgery for colorectal cancer. However, it seemed to be an effect of perioperative trauma. Further studies are needed to investigate the impact of surgical intervention on lymphocyte subpopulations.

Keywords

Lymphocyte subsets; T-lymphocytes, regulatory; Th17 cells; Lymphocyte count; Colorectal surgery

Hrčak ID:

224608

URI

https://hrcak.srce.hr/224608

Publication date:

1.6.2019.

Article data in other languages: croatian

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