Short communication, Note
https://doi.org/10.18054/pb.v120i2-3.6749
The effect of subminimal inhibitory concentrations of antibiotics on the adherence ability of Pseudomonas aeruginosa to epithelial cells in vitro
Sanja Bekić
; Sanja Bekić, MD, MSc, Family Medicine Practice, Osijek, Croatia; Department of Internal Medicine, Family Medicine and History of Medicine, Faculty of Medicine, University of Osijek, Osijek, Croatia
Domagoj Drenjančević
; Department of Transfusion Medicine, Osijek University Hospital, Osijek, Croatia; Department of Microbiology and Parasitology, Faculty of Medicine, University of Osijek, Osijek, Croatia
Jasminka Talapko
; Department of Microbiology and Parasitology, Faculty of Medicine, University of Osijek, Osijek, Croatia; Faculty of Dental Medicine and Health, University of Osijek, Osijek, Croatia
Ivan Kotris
; Department of Internal Medicine, General County Hospital Vukovar, Vukovar, Croatia
Ivana Škrlec
orcid.org/0000-0003-1842-930X
; Faculty of Dental Medicine and Health Osijek
Jasmina Vraneš
orcid.org/0000-0002-3282-0973
; Department of Microbiology and Parasitology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Microbiology, Andrija Stampar Teaching Institute of Public Health, Zagreb, Croatia
Abstract
Background and purpose: The aim of this study was to examine the influence of subminimal inhibitory concentrations (subMICs) of ceftazidime, ciprofloxacin and gentamicin on the adherence ability and morphology of wild-type Pseudomonas aeruginosa strains to the Buffalo green monkey kidney cell line, using indirect immunofluorescence staining.
Materials and methods: Bacterial adherence changes were tested before and after exposure to 1/2, 1/4, 1/8, 1/16 and 1/32 MIC of antibiotics.
Results: A statistical difference in the number of attached bacteria after exposure to all subMICs of ceftazidime and ciprofloxacin was observed (p<0.05), even after only 1/2 MIC of gentamicin.
Conclusion: The results of this study have shown that antibiotics in much lower concentrations than those necessary for inhibition of bacterial multiplications could damage the adherence of Pseudomonas aeruginosa to the epithelial cell line.
Keywords
Ceftazidime; Ciprofloxacin; Gentamicin; Bacterial adherence
Hrčak ID:
225311
URI
Publication date:
16.9.2019.
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