Original scientific paper
https://doi.org/10.20302/NC.2019.28.22
DNA barcoding of the family Phryganeidae (Insecta, Trichoptera) in Croatia with particular reference to phylogeny, distribution and conservation biology
Mladen Kučinić
; Department of Biology (Laboratory for Entomology), Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
Anđela Ćukušić
; Croatian Ministry of Potection of Nature and Energetic, Radnička cesta 80/7, 10000 Zagreb, Croatia
Darko Cerjanec
; Primary school Barilović, Barilović 96, 47252 Barilović, Croatia
Martina Podnar
; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Mladen Plantak
; Elektroprojekt d.d., Civil and Architectural Engineering Department, Water Resources, Nature and Environmental protection, Alexandera von Humboldta 4, 10000 Zagreb, Croatia
Sanja Žalac
; ZSC „Dr. Ivo Pevalek“, National park Plitvice Lakes, Josipa Jovića 19, 53231 Plitvička jezera, Croatia
Renata Ćuk
; Hrvatske vode, Ulica grada Vukovara 220, 10000 Zagreb, Hrvatska 10000 Zagreb, Croatia
Ivan Vučković
; Elektroprojekt d.d., Civil and Architectural Engineering Department, Water Resources, Nature and Environmental protection, Alexandera von Humboldta 4, 10000 Zagreb, Croatia
Halil Ibrahimi
; Department of Biology, Faculty of Mathematics and Natural Sciences, University of Prishtina, Mother Theresa p.n., 10000 Prishtina, Kosovo
Antun Delić
; Nikole Šubića Zrinskog 3, 43290 Grubišno Polje, Croatia
Abstract
In Europe the Phyrganeidae family comprises 7 genera and 20 species. In Croatia, 6 species have been recorded from this family, and four of them recorded and collected in the last five years have been DNA barcoded. In terms of faunistic research, most interesting is the record of Trichostegia minor Curtis, 1834 from the region of the Gacka River in Lika, in upland Croatia. Up to now four species have been included in the BOLD system with 6 DNA-barcoded specimens. From the Gacka River area, 10 specimens of 8 species, including T. minor, have been barcoded and included into the BOLD system.
Phylogenetic research based on DNA barcode data has shown some interesting results. Specimen of T. minor collected in the area of the Gacka River is clustered with a specimen of this species from Austria into a separate subgroup, which shows minor molecular specifics. Phylogenetic analysis has confirmed the justification of the status of a subspecies from Mongolia, Phryganea grandis rotundata Ulmer, 1905.
Future Trichoptera fauna research in Croatia will continue investigations of the family Phryganeidae in various parts of the country for the purpose of ascertaining the detailed distribution of all the species recorded, to enable finding some other previously missing species, as well as collecting and
DNA barcoding of Hagenella clathrata (Kolenati, 1848) and Oligostomis reticulata (Linnaeus, 1761). With respect to these species, the data will also be useful for the area of conservation biology, necessary for their protection and for the protection of the sites in which they occur, for it is clear that these are very rare species in Croatia, T. minor also belonging to this group: 50% of the species from this family recorded in Croatia are rare and very rare species.
Keywords
COI; Phryganea grandis rotundata; Trichostegia minor; south-east Europe; dinaric karst; the River Gacka
Hrčak ID:
231148
URI
Publication date:
31.12.2019.
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